Photo bioreactor/Fermenter/Fermentation tank/yogurt fermentation tank/Industry pilot fermentor

No

Item

Description

1

Vessel system

Autoclavable bioreactor vessel, 10 liter (5 to 7.5 liter working volume) total capacity  ,Stainless steel 316L

With sterile and pressure proof ports

Fermentor with borosilicate vessel with total volume of 2 liters and working volume from 2 Liter to 3.5 Liter FERMENTOR Vessel must be a Jacketed Vessel having with the head plate of SS316L. It should have the Temperature Control via circulation of Hot / Cold Water in the Jacket through an inbuilt Thermo circulator unit (working temperature range 8ºC - 80ºC above coolant temperature) The system must be complete to measure and control pH, DO, stirrer speed (20-1000rpm), foam control & temperature.

Agitation

Mechanical seal, shaft, 2 height adjustable six – blade stirrer, removable baffle cage

Aeration

Auto cleavable sterile filter, check valve, connection piece, aeration tube with ring sparser

Required air pressure approx, 1.5 bar

Ventilation

Reflux cooler, connection piece, auto cleavable sterile filter

Temperature Circuit

Connected to glass double jackets, all four temperature circuits connected by central supply and drain lines.

Probes

Autoclavable temperature probe Pt100 incl. connecting cable

Autoclavable pH-gel-electrode incl. connecting cable

Autoclavable pO2-probe incl. connecting cable

Autoclavable height adjustable antifoam-probe incl. connecting cable

Sampling System

Autoclavable sampling system

Inoculum

Cup for silicone diaphragm

Measurement of Controls

Integrated in stainless steel trunking

Power supply

Main power supply for all electrical circuits, 1 x 220V, 50 Hz

Temperature Control

Including heating finger and cooling water valve in temperature circuit. Temperature controller for heating and cooling, temperature range 4-60C (depending on the available cooling media)

Speed Setting

DC motor with frequency converter, rpm-control unit

pH Control

pH-control to activate the dosage of acid and base (pumps)

pO2 Control

DDC pO2-control to activate the final elements, Cascade control vial stirrer speed and aeration valve

Antifoam Control

DDC antifoam - Control to activate the dosage to antifoam chemicals

Dosage

Aeration Unit

Consists of pressure valve, a rotameter (50-500L/h) a continuous solenoid valve (proportional valve), activated by pO2 controller, a check valve.

Peristaltic Pump with variable speed

2

Aeration system

Include rotameter, aeration filter

3

Agitation system

Magnetic Drive from bottom, without mechanical seal
The agitation system hanged up is driven by magnetic force. There is no thimble at the bottom. The rotate speed is steady. High-powered stirrer, antifoam stirrer, AC buncher, variable speed control. All parts in the cylinder are set on the cover, so it is convenient to maintain and clean. Standard six- bladed paddle used in fermentation process can be adjusted in the height. The stirrer which has many other styles can be used to meet the special requirement of fermentation process. There is no possibility to leak out the bacteria without mechanical sealing. AC buncher, actiyator. The design which separates the motor from the cylinder makes the weight of cylinder cut down, so it is convenient to operate and save a large amount of manpower. The magnetic force system uses special permanent magnetic material. The special process makes the magnetism permanent. The highest rev will never be less than 980 rpm during the service life of the cylinder. It can meet the highest rev even in the full bodied medium.

4

Temperature control

Interlayer electric calefaction, auto-control

5

Airflow control

Provision for mixing at least four gases, necessary rotameters, valves, gas mix station to be included. Air filtration with membrane filter Supply capacity for fermenter: Air: up to 2 VVM, Oxygen ≥ 0.5 VVM, Sparser – One number sparser.

6

Antifoam control

Automatically check foam, automatically add antifoam by peristaltic pump.

7

Feeding control

Control by peristaltic pump switch, automatically feeding and computation.

8

pH control

Automatically add acid, alkali by peristaltic pump and control pH accurately.

9

DO control

Control with rotating speed relatively.

10

Illumination system

 LED light (white, blue, green, red)  with adjustable intensity available (intensity 0 until 50micromol-m.seg2) and photoperiod

11

BLBIO-B Biology process controller

Control parameter: temp, pH, DO, antifoam, stirrer rev, feeding; (Other parameters like glucose, amino acid, ethanol, oxygen, carbon dioxide, electric balance system can be chosen to use.)

12

Control system

BLBIO B Control System: on-site controller, the next crew for the Siemens S7-200 series PLC control system, it is a mature, stable, universal type of PLC, to suit a variety of automation applications, especially in manufacturing process control Application. Its modular, easy to implement distributed configuration and easy to master and so on, Siemens PLC control center has become a biological reaction process control implementation is economical and advanced control system, the system uses the touch screen display, on-site direct operations (menu type), all English menu and interface; also with the host computer for data transmission, data sampling and display tank, the system view of data analysis, reading settings.

10-inch LCD touch-sensitive screen as the display screen to display complete content-rich, user-friendly screen is simple, easy to operate. And disturbing each other. The control system can adapt to different range of sensors and actuators the output of the input signal with the signal anti-jamming system.

Software: Siemens S7+  FORCE  CONTORL

Data collection and control module: Siemens S200 PLC controller and data collection and control module

1. Control
1) Manual control: You can set the percentage of open valve or a valve opening  2) automatic control mode: You can select continuous or PID PID control the switch ,3) sequence control: the control of all parameters can be pre-set at least 10 control section, 4) for automatic segmentation remotely control: PC control ,5) acceptable correlation control: the dissolved oxygen can select speed, air flow rate, tank pressure, control of feeding, etc.; pH can choose to control with acid and alkali

2. Measurement function: can feeding, bubble enemy, such as measurement of pH
3. XY-axis to chart real progress can be any expansion and contraction of graphics display, to help the operator determine the parameters of the interaction between the effects of the fermentation process to quickly modify and adjust

4. Data Processing
1) to show that all the parameters of the trend curve analysis
2) to store, display, analyze all the historical curve parameters
3) can display all the parameters of the batch report
4) copy the data directly with the USB

5) Data storage format is compatible with EXCEL, EXCEL platform can be to deal with
5. Controller self-protection function: to set the password, others can not modify the fermentation parameters; and the controller is not lost due to power settings for each parameter
6. Can the process control parameters, each parameter can have 10 block can be set
7. Time display of each parameter recording interval can be adjusted
8. Two parameters can be carried out with the screen settings and calibration

9. show the controller is really running against, the panel instructed the state process system, process hardware, and motion status indicator for understanding the various devices (such as peristaltic pumps, circulating pumps, heating, mixing, etc.) work status, Fault identification can be used as display
10. Each parameter of the transmission are independent modules, without disturbing each other, for easy maintenance
11.PH, DO and other parameters of control and associated controls can be adjusted

13

PC software

BLBIO-Ver8.0 is a data recording and processing as one of the fermentation of special packages, with the Chinese operation interface, data storage and backup, curve analysis, report generation analysis, anomaly analysis, for the fermentation and production process control and data processing bring a lot of convenience.
Main features:
1. Can record the fermentation time, temperature, pH, dissolved oxygen, rotational speed, air flow, pressure; fill quantity, the amount of foam enemy, acid dosage
2. Can be sent under the controlled variable settings and control parameters, remote operation
3. Can be set sequence control
4. Can type off-line parameters, and records show
5. Trend curve, historical curve shows
 can display 1-10 (any setting) curve;
- can select a different start time and the time period (8,24,48,72,96 and 144 hours);
- can set the upper and lower curves show, in order to improve display resolution;
- can choose any of several different batches (1-10) curve also shows that for comparative analysis;
- and Y-axis parallel to the secant by the mouse to drag the reading, followed by curve data is activated, the fermentation time (X axis) and the same time the data curve with the secant movement changes;

 6. Report Printing
- can show significant 1-12 (any set) data, and print output;
- You can select a different start time and the time and can choose different time intervals (1,5,15,30,60 minutes);
- You can choose any of several different batches (any set) data, and print output,
- To comparative analysis;

 7. curve printing
- You can show the curve was printed by color printer;
EXCEL platform can also be carried out in data processing and printing, is very convenient.